Two Pairs of Isomerically New Phenylpropanoidated Epicatechin Gallates with Neuroprotective Effects
 
作者: 文章来源: 点击数: 更新日期:2019-05-05
 

Two Pairs of Isomerically New Phenylpropanoidated Epicatechin Gallates with Neuroprotective Effects on H2O2
?Injured SH-SY5Y Cells from Zijuan Green Tea and Their Changes in Fresh Tea Leaves Collected from Different Months and Final Product

Authors

Jia-Ping Ke, Wen-Ting Dai, Wen-Jun Zheng, Hao-Yue Wu, Fang Hua, Feng-Lin Hu, Gang-Xiu Chu*, Guan-Hu Bao*

Journal

Journal of Agricultural and Food Chemistry

DOI

10.1021/acs.jafc.9b01365

Abstract

Zijuan tea (Camellia sinensis var. assamica), an anthocyanin-rich cultivar with purple leaves, is a valuable material for manufacturing tea with unique color and flavor. In this paper, four new phenylpropanoid substituted epicatechin gallates(pECGs), Zijuanins A-D (1-4), were isolated from Zijuan green tea by different column chromatography. Their structures were identified by extensive high resolution mass spectroscopy (HR-MS), nuclear magnetic resonance (NMR), and experimental and calculated circular dichroism (CD) spectroscopic analyses. Detection of the changes in fresh tea leaves collected from April to September and the final processed product by high performance liquid chromatography (HPLC)-HRMS suggested that production of compounds 1 and 2 may be enhanced by the processing procedure of Zijuan green tea. Additionally, 1-4 were proposed to be synthesized through interaction between the abundant secondary metabolite ECG and phenolic acids from tea leaves by two key steps of phenol-dienone tautomerism. 1 and 2 showed impressive activity in protecting SH-SY5Y cells against H2O2-induced damage at the concentration of 1.0 μM.
 



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