Genome-Wide Analysis of the Biosynthesis and Deactivation of Gibberellin -Dioxygenases Gene Family
 
作者: 文章来源: 点击数: 更新日期:2017-09-26

Title


Genome-Wide Analysis of the Biosynthesis and Deactivation of Gibberellin -Dioxygenases Gene Family in Camellia sinensis (L.) O. Kuntze


Authors


Cheng Pan†, Kunhong Tian†, Qiuyan Ban, Leigang Wang, Qilu Sun, Yan He, Yuanfei Yang, Yuting Pan, Yeyun Li, Jiayue Jiang, Changjun Jiang*


Journal


Genes 2017, 8, 235; doi:10.3390/genes8090235


Abstract


Gibberellins (GAs), a class of diterpenoid phytohormones, play a key role in regulating diverse processes throughout the life cycle of plants. Bioactive GA levels are rapidly regulated by Gibberellin-dioxygenases (GAox), which are involved in the biosynthesis and deactivation of gibberellin. In this manuscript, a comprehensive genome-wide analysis was carried out to find all GAox in Camellia sinensis. For the first time in a tea plant, 14 CsGAox genes, containing two domains, DIOX_N (PF14226) and 2OG-FeII_Oxy, were identified (PF03171). These genes all belong to 2-oxoglutarate-dependent dioxygenases (2-ODD), including four CsGA20ox (EC: 1.14.11.12), three CsGA3ox (EC: 1.14.11.15), and seven CsGA2ox (EC: 1.14.11.13). According to the phylogenetic classification as in Arabidopsis, the CsGAox genes spanned five subgroups. Each CsGAox shows tissue-specific expression patterns, although these vary greatly. Some candidate genes, which may play an important role in response to external abiotic stresses, have been identified with regards to patterns, such as CsGA20ox2, CsGA3ox2, CsGA3ox3, CsGA2ox1, CsGA2ox2, and CsGA2ox4. The bioactive GA levels may be closely related to the GA20ox, GA3ox and GA2ox genes. In addition, the candidate genes could be used as marker genes for abiotic stress resistance breeding in tea plants.




相关附件
  • genome-wide analysis of the biosynthesis and deactivation of gibberellin (潘铖,田坤红,江昌俊,2017).pdf
  • CopyRight (C) 2015 版权所有 茶树生物学与资源利用国家重点实验室

    中国·安徽·合肥·长江西路130号 230036; Tel:+86-551-65786401;65786701; Fax:+86-551-65786765