Construction and characterization of a bacterial artificial chromosome library for Camellia sinensis
 
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Title


Construction and characterization of a bacterial artificial chromosome library for Camellia sinensis


Authors


Yuling Tai†, Hao Wang†, Chaoling Wei, Ling Su, Min Li, Liqun Wang, Zhaozhao Dai, Lingyun Shao, Jiayue Jiang, Meizhong Luo, Hua Yang, Weiwei Deng, Bei Huang, Xiaochun Wan*, Jun Sun*


Journal


Tree Genetics & Genomes (2017) 13: 89 DOI 10.1007/s11295-017-1173-5


Abstract


Tea is a popular and natural non-alcoholic beverage, and is produced from fresh leaves of
Camellia sinensis. Tea leaves contain many bioactive compounds that have significant health benefits. We constructed a high quality bacterial artificial chromosome (BAC) library by using the fresh petals of C. sinensis “Shuchazao” for genome sequencing and improvement of genomic assembly. BAC library is still a significant tool for studies of functional genomes and preservation of precious genetic resources. The BAC library contains 161,280 clones with an average insert size of 113 kb, which represents approximately 6.2-fold coverage of haploid genome equivalents of C. sinensis. We characterized 20 complete BAC clones and 738 BAC end sequences (BESs) ranging from 105 to 917 bp. In addition, we predicted cisregulatory elements of LAR (leucoanthocyanidin reductase), TCS (caffeine synthase), and TS (theanine synthetase) involved in tea characteristic metabolite synthesis and identified a larger number of light-responsive cis-acting elements in these three genes. Meanwhile, we analyzed alternative splicing of these three genes. Furthermore, 12 pairs of SSR primers were successfully amplified in tea plant DNA. The tea BAC library was a critical resource to accomplish de novo wholegenome sequencing, accelerate gene discovery and enhance molecular breeding of C. sinensis.




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  • construction and characterization of a bacterial artificial(邰玉玲、王浩、宛晓春、孙俊,2017).pdf
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