Novel acetylcholinesterase inhibitors from Zijuan tea and biosynthetic pathway
 
作者: 文章来源: 点击数: 更新日期:2017-07-01

Title

Novel acetylcholinesterase inhibitors from Zijuan tea and biosynthetic pathway of caffeoylated catechin in tea plant

Authors

Wei Wang#, Xiwen Fu#, Xinlong Dai#, Fang Hua, Gangxiu Chu, Mingjie Chu, Fenglin Hu, Tiejun Ling, Liping Gao, Zhongwen Xie, Xiaochun Wan, Guanhu Bao *

Journal

Food Chemistry 237 (2017) 1172–1178

Abstract

Zijuan tea is a special cultivar of Yunnan broad-leaf tea (Camellia sinensis var. assamica) with purple buds, leaves, and stems. Phytochemical study on this tea led to the discovery of three hydroxycinnamoylated catechins (HCCs) (1–3), seven other catechins (4–10), three proanthocyanidins (11–13), five flavones and flavone glycosides (14–18), two alkaloids (19, 20), one steroid (21), and one phenylpropanoid glycoside (22). The isolation and structural elucidation of the caffeoylated catechin (1) by means of spectroscopic techniques were described. We also provide the first evidence that 1 is synthesized via a two-step pathway in tea plant. The three HCCs (1–3) were investigated on their bioactivity through molecular modeling simulation and biochemical experiments. Our results show that they bind acetylcholinesterase (AChE) tightly and have strong AChE inhibitory activity with IC50 value at 2.49, 11.41, 62.26 µM, respectively.

 




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  • novel acetylcholinesterase inhibitors from zijuan tea (王威,付希文,代新龙,鲍官虎,2017).pdf
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