First Report of Leaf Blight Caused by Arthrinium arundinis on Tea Plants in China
 
作者: 文章来源: 点击数: 更新日期:2019-11-29

Title


First Report of Leaf Blight Caused by Arthrinium arundinis on Tea Plants in China


Authors


Kuberan Thangaraj, Lin-Lin Cheng, Cheng Deng, Wei-Wei Deng, Zheng-Zhu Zhang


Journal


Plant Disease


https://doi.org/10.1094/PDIS-06-19-1324-PDN


Abstract


Tea (Camellia sinensis [L.] Kuntze) is one of the most important economic crops in China. During a survey in July 2018, symptoms of brown leaf patches (3 to 4 cm diameter) with irregular margins were observed on tea leaves of Longjing43 cultivar in Huoshan tea farm, Anhui province, China. The disease incidence reached 18 to 20% in the most severely affected tea gardens. Lesions first appeared as gray-brown dots, subsequently expanding to irregular dark brown, and then ended with large brown patches on the tea leaves. The infected tea leaf samples were collected from the tea plants, and the pathogenic fungus isolation was performed according to the method described by Thangaraj et al. (2018). Briefly, the tea leaf samples washed with tap water followed by distilled water. The margins of infected leaves were cut into small pieces (4 to 5 mm2) and surface sterilized with 0.1% HgCl2 for 1 min followed by 70% ethanol for 30 s and rinsed three times in sterile distilled water. Then, these aseptic tissues were blotted dry on sterilized filter paper, transferred onto 2% water agar, and incubated in the dark at 28°C until fungal hyphae started to grow. Single hyphal tips were transferred onto potato dextrose agar (PDA) plates and incubated for 7 days at 28°C for morphological observations. A total of five isolates were obtained and identified as the same fungus on the basis of phenotypic characteristics. The colonial characters of the isolated fungus on PDA were dirty white and moderate aerial mycelium with dark colored patches.


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