Phyllosticta capitalensis Causes Leaf Spot on Tea Plant (Camellia sinensis) in China
 
作者: 文章来源: 点击数: 更新日期:2019-11-29

Title


Phyllosticta capitalensis Causes Leaf Spot on Tea Plant (Camellia sinensis) in China


Authors


Cheng,Lin-Lin;  Thangaraj, Kuberan;   Deng, Cheng;   Deng, Wei-Wei;  Zhang, Zheng-Zhu


Journal


Plant Disease


DOI  


10.1094/PDIS-04-19-0768-PDN


Abstract


Tea plants are on China’s important economic crops list. A new fungal infection started with small lesions with reddish brown edges, and it extended until numerous visible pycnidia were observed on the mature leaves of 10-year-old tea plant in Chizhou, Anhui Province, China, in September 2018. To isolate the causal agent, symptomatic leaves were randomly collected from the tea garden and were washed with tap water, followed by distilled water. The margins of infected leaves were cut into small pieces (4 to 5 mm2), surface sterilized with 0.1% HgCl2 for 1 min followed by 70% ethanol for 30 s, and rinsed three times in sterile distilled water (Chen et al. 2016). Then, the pieces were blotted dry on sterilized filter paper, placed on 2% water agar, and incubated in the dark at 28°C until fungal hyphae started to grow. Single hyphal tips were transferred onto potato dextrose agar (PDA) plates and incubated for 10 days at 28°C for phenotypic observations. Morphology of the colonies was examined on PDA; observations showed dark green mycelium with granular surface and uneven edges with white later turning black and black globose pycnidia.


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